Search parameters: the BLAST E-value threshold was set at 1e-05. The Pfam and TIGRFAMs threshold was set at the trusted cutoff for each model. Enzyme Catalog references were obtained from PRIAM using a search cutoff of 1e-20. COGs was run using the default cutoff. tRNAscan-SE was run using the Prokaryotic default settings. Gene model numbers refer to the combined list, not the specific method.

The BLAST btab output files may need a header line to define the columns.